Category: 4 No firm DNA hoax

Wednesday, April 22, 2009

Understanding Why The DNA Is On The Knife

Posted by Peter Quennell

[click for larger image]




Our DNA poster Nicki has been careful not to exaggerate the impact as evidence of the DNA on the knife found in Sollecito’s apartment.

She accepts that in the eyes of the court there could be question marks over the size of the sample and the fact that the tests could not be repeated.

However, as the knife appeared to have been thoroughly cleaned with bleach, some remain intrigued that any DNA at all was found.

Here is a short piece explaining why. This article by Juliet Lapidos was posted on the Slate site in November 2007. But we haven’t seen better, and it is still often referred to.

Slate 20 Nov 2007
How To Clean a Bloody Knife: Does DNA come off with soap and water?
By Juliet Lapidos NYTimes Staff Writer

Investigators in Perugia, Italy, have found new evidence linking a 20-year-old American exchange student, Amanda Knox, to the brutal stabbing death of her roommate, British student Meredith Kercher. According to the latest reports, Knox and her Italian boyfriend, Raphael Sollecito, cleaned the alleged murder weapon””an 8-inch black-handled kitchen knife””with bleach. Nevertheless, police discovered Kercher’s DNA on the tip and Knox’s DNA by the handle. Is it possible to clean DNA off a knife?

Yes, if you know what you’re doing. Knox and Sollecito were on the right track: Bleach contains sodium hypochlorite, an extremely corrosive chemical that can break the hydrogen bonds between DNA base pairs and thus degrade or “denature” a DNA sample. In fact, bleach is so effective that crime labs use a 10 percent solution (one part commercial bleach to nine parts water) to clean workspaces (PDF) so that old samples don’t contaminate fresh evidence. Likewise, when examining ancient skeletal remains (PDF), researchers first douse the remains in diluted bleach to eliminate modern DNA from the surface of bones or teeth.
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So, why did Knox and Sollecito’s bleaching gambit fail? It’s difficult to swab a knife thoroughly. Dried blood can stick to the nooks and crannies in a wood handle, to the serrated edge of a blade, or become lodged in the slit between the blade and the hilt. With help from a Q-tip, it’s possible to eliminate most stains, but what’s not visible to the naked eye might still be visible to a microscope, and sophisticated crime labs need only about 10 cells to build a DNA profile.

Bleach is perhaps the most effective DNA-remover (though evidently no methodology is failsafe), but it’s not the only option. Deoxyribonuclease enzymes, available at biological supply houses, and certain harsh chemicals, like hydrochloric acid, also degrade DNA strands. It’s even possible to wipe a knife clean of DNA-laden hair follicles, saliva, and white blood cells with generic soap and warm water. The drawback to this last method is that the tell-tale cells don’t just disappear once off the knife. They linger on sponges, in drains, and even in sink traps, where wily investigators search for trace evidence.

There appears to be a great deal more DNA evidence than merely what is on the knife, of course, and early in the trial the known luminol-evidence universe also expanded.

The court was told that AK-sized and RS-sized footprints appeared under luminol on the floor of Filomena’s room.

Nicki’s two Powerpoints on the DNA can be seen here and here and Kermit’s Powerpoint (pre the new evidence) on the luminol can be seen here.

Wednesday, February 25, 2009

Understanding Micheli #4: The Faked Crime Scene - Who Returned To Move Meredith?

Posted by Brian S

Here now is the full 2011 Micheli Report kindly translated by Catnip for the Wiki and TJMK.

1. Where We Stand

Just to recap. Judge Micheli presided over Rudy Guede’s trial and sentencing and the final hearing that committed Raffaele Sollecito and Amanda Knox to trial.

Late January he made public the 106-page report that explains the thinking behind both actions. These posts are examining key areas of the report so that we too may decide on the rationales.

2. The Final Position Of The Body

Why this matters so much is that if the evidence holds firm, all by itself it will prove that there was a major rearrangement of the crime scene, to try to throw investigators off the trail.

This is as near to an 80,000 pound gorilla in the room as we are likely to see in this trial. And it may even be on the trial agenda for this coming Friday and Saturday.

Reports by the crime-scene investigators and Dr Lalli are summarised in Judge Micheli’s report. They describe the detail of the scene discovered in Meredith’s room. The investigators measured and photographed the position and state of everything, including blood, as it was in the room before anything was moved.

Amongst the items noted was a white bra. Some parts were soaked in blood, particularly the right shoulder strap and the outside of the left cup. They also noted that a portion of the backstrap with its clasp fixings was missing. Meredith herself was lying on her back midway between the wardrobe and the bed, without her jeans, a pillow under her buttocks and her top rolled up to reveal her chest.

Following this survey, Meredith’s body was then turned and moved by the investigators. This revealed the other items on which her body had lain. A tennis shoe, a white sheet from the bed and a blue zipped top, all with blood stains. Also a green bath towel and an ivory bath towel, both soaked in blood, and underneath the pillow was the missing clasp section of the bra back-strap.

Judge Micheli notes that Amanda’s defence claimed that “the small round spots of blood” apparent on Meredith’s chest indicated that she was not wearing her bra when she was killed. He agreed that it was likely that these spots fell from Meredith’s gasps for breath as she lay on her back after she had been stabbed. However, he could not agree with their conclusion that her bra had been removed before this time, as similar small round spots were also found on Meredith’s bra.

Micheli reasoned that this indicated that Meredith was still wearing her bra as she gasped for breath, but that her top was rolled up and the bra moved also. Thus indicating the sexual nature of the original attack, but also allowing the small round spots to fall on both chest and bra. Furthermore, other blood evidence involving the bra indicated that it wasn’t removed until some time after Meredith had died.

He said that Meredith’s bra was found by investigators away from other possible blood contamination on the floor, near to her feet. Photographs of Meredith’s body show clear white areas where the bra prevented blood from falling onto Merediths body. These white areas corresponded to those areas where blood was found on her bra. This was particularly true in the area of the right shoulder strap which was soaked from the wound to Meredith’s neck.

Micheli said that evidence showed that Meredith had lain on one shoulder near the wardrobe. She lay in that position long enough for the imprint of her shoulder and bra strap to remain fixed in the pool of blood after she was moved to the position in which her body was finally found. Photographs of blood on her shoulder matched the imprint by the wardrobe and her shoulder itself also showed signs that she had remained in that position for some time.

Based on all this, Judge Micheli concluded that there could be no doubt that Meredith’s body was moved away from the wardrobe and her bra removed quite some time after her death.

Neighbor Nara Capezzali had testified that people fled from the cottage within a minute of Meredith’s final scream. There was no time for any alteration of the crime scene in those very few moments.

Judge Micheli asks in his report, who could have returned later and faked the scene which was found? Who later moved Meredith’s body and cut off her bra? He reasons it could only be someone who had an interest in changing what would become a crime scene found at the cottage. Who else but someone who lived there, and who wanted to mislead the coming investigation?

It couldn’t have been Laura, she was in Rome. It couldn’t have been Filomena, she was staying with her boyfriend. It was very unlikely that it was Rudy Guede, all proofs of his presence were left untouched.

The culprits ran from the cottage in different directions and there is no reason to believe they met up again before some or one of them returned. Judge Micheli stated that, in his opinion, this just left Knox who would seem to have an interest in arranging the scene the police would find.

Bloody footprints made visible with luminol in Filomena’s room contain Meredith’s DNA. This indicated to Judge Micheli that the scene in Filomena’s room was also faked after Meredith was killed.

In Micheli’s opinion the scene in Meredith’s room was probably faked to point the finger at Rudy Guede. All evidence related to him was left untouched, and the pillow with a partial palm print was found under Meredith’s repositioned body.

But whoever later arranged that scene in Meredith’s room also unwittingly indicated their own presence at the original sexual assault. Who else could have known that by staging an obvious rape scene, they would inevitably point the investigators towards Rudy’s DNA which they knew could be found in Meredith?

Micheli asks: Seemingly, who else could it have been but Amanda Knox? And this in part is why she was committed to trial, for her defense to contend this evidence.

Monday, February 23, 2009

Understanding Micheli #3: Precisely How Damning Is The DNA Evidence?

Posted by Nicki

Here now is the full 2011 Micheli Report kindly translated by Catnip for the Wiki and TJMK.

1. The Context

Judge Micheli has had two very important roles. He presided over Rudy Guede’s trial and sentencing, and he presided over the final hearing that committed Raffaele Sollecito and Amanda Knox to trial.

Late January, Judge Micheli made public the 106-page report that explains the thinking behind both actions.

These four posts are examining several very key areas of the report so that we too may choose whether to buy into the rationales. I recommend watching this Powerpoint.

Click for Post:  Powerpoints #7: DNA Evidence - A Very Clear Intro To A Vital Subject Here

2. The DNA Evidence

Coming up soon is a more silent witness, one very important to both the prosecution and the two defenses: the extensive DNA evidence.

Specifically the DNA belonging to Meredith, Knox, Sollecito, and Guede, which was found at the scene of the crime, and on the suspected murder weapon found, apparently hidden, in Raffaele Sollecito’s house.

DNA of Sollecito has been found on the clasp of the victim’s bra. DNA of Amanda Knox was identified on the knife handle and also in the bathroom and Filomena’s room. DNA of Meredith DNA has been found on a knife compatible with the wounds that caused her death. DNA of Guede was found on the victim’s body and items and elsewhere in the house.

In summary, the biological sources and locations where DNA belonging to the victim and three defendants was found are these:

Guede’s DNA

DNA (from epithelial cells) was found inside Meredith, also on toilet paper, also on the right side of Meredith’s bra, also mixed with Meredith’s DNA on the her purse zip, and also on the left cuff of Meredith’s light blue sweater. Five total.

Sollecito’s DNA

DNA (from epithelial cells) was found on Meredith’s bra clasp, mixed with Meredith’s DNA, and also on one cigarette butt found in the kitchen. Two total.

Knox’s DNA

DNA (from epithelial cells) was found on the knife handle, and also close to the blade junction. DNA was also found in the small bathroom and in Filomena’s room

Meredith’s DNA

DNA was found on the knife blade. It was not possible to ascertain both the haematic and epithelial source of Meredith’s DNA on the knife blade, due to the scarcity of the sample. Numerous significant biological traces belonging to Meredith were found outside her bedroom - for example, DNA originating from the blood-trace footprints revealed by luminol found in Filomena’s bedroom, as reported at the Massei trial. Many instances total.

3. Defence Claims

Claims of contamination and “poor matches” of the DNA samples were raised by the Sollecito and Knox defenses, although not by Guede’s.

These claims had not been raised by the defenses’ experts who observed all the testing. 

4. Prosecution Rebuttal

The DNA expert Dr. Stefanoni’s arguments in reply to the defenses’ claims are summarized in Judge Micheli’s report.

Low Copy Number

Dr Stefanoni reported that the locus ascribable to Meredith and identified on the knife blade shows readings of 41 and 28 RFU.

Conventionally, RFU values lower than 50 can be defined as low. But she maintained that the profile matched Meredith’s by explaining that there is no immediate correlation between the height of the peaks obtained by electropherogram and expressed in RFU, and the reliability of the biological investigation.

In fact even if statistically - in most cases - the RFU data is directly proportional to the possibility of a certain interpretation of the analysis result, on the other side many cases of high peaks of difficult interpretation exist (because of background noises), as well as low peaks that are objectively unquestionable, hence the need to proceed to the examination of data that is apparently scarce, but that mustn’t be considered unreliable per se.

The use of multiplex PCR and fluorescent dye technology in the automated detection and analysis of short tandem repeat [STR] loci provides not only qualitative information about the profile - i.e. which alleles are present - but can provide also quantitative information on the relative intensities of the bands, and is therefore a measure of the amount of amplified DNA.

So if on one side Dr Stefanoni admits that the RFU readings are low, on the other her experience suggests that many cases of unquestionable matches exist showing readings lower than 50 RFU, and this appears to be the case with Meredith’s DNA sample on the knife.

Possibility Of Contamination

Contamination in the laboratory is categorically excluded by Dr Stefanoni. The samples were processed with maximum care in order to avoid any contamination during lab procedures. Defense experts observed all processing and nevr raised any objection.

Contamination during the collection phase is excluded by Judge Micheli, as the samples were collected by different officers at different times in different places (example Via della Pergola at 9:40am on Nov 6. 2007, and Sollecito’s apartment at 10:00am, on the same day, by a different ILE team).

As for Sollecito’s DNA found on the bra clasp, the match is unquestionable, according to the lab reports.

Samples from crime scenes very often contain genetic material from more than one person (e.g. Rudy Guede’s DNA has been identified in a mixture with the victim’s DNA in a few places), and well-known recommendations and protocols exist in order to de-convolute mixed samples into single genetic profiles.

So if the lab reports indicate that unquestionable biological evidence of Sollecito’s DNA was found on the bra clasp, at the present time we have no reason to believe that these recommendations weren’t followed and that therefore the reports are not to be trusted.

Conspiracy Theories

As to cells “flying around” depositing themselves - and their DNA content - here and there around the murder scene, there have been some imaginative theories advanced, to say the least.

The reality though is that although epithelial cells do shed, they don’t sprout little wings to flock to one precise spot, nor grow feet to crawl and concentrate on a piece of evidence.

There needs to be some kind of pressure on a surface in order to deposit the amount of biological material necessary to yield a reliable PCR analysis result.

A simple brushing will not do. 

As a matter of fact, Dr Stefanoni agreed with Guede’s defense that Guede’s genetic material found on the left sleeve of Meredith’s blouse was minimal; and this was because the DNA found there belonged to the victim and was not a mixture.

In the situation where there is a clear disproportion between quantitative data of two DNA’s coexisting in a biological trace, the PCR will amplify the most abundant DNA.

As agreed by Dr. Stefanoni and Guede’s defense, the conclusion here was that on the left sleeve there was plenty of Meredith’s DNA; but very little of Guede’s. This was used by his defense to deny that Guede had exerted violence on Meredith’s wrist.

5. Judge Micheli’s Ruling

After listening to the arguments of the prosecution and the defenses, Judge Micheli provided reasons why he rejected the contamination claims and ruled that all the biological traces identified as reflecting Sollecito’s and Knox’s DNA are admissible as evidence.

He arrived at the conclusion that the DNA evidence is sound and, considered along with the non-biological proof, he decided there was more than enough evidence to order Knox and Sollecito to stand trial.

6. Relevance To 2009 Trial

Regarding the biological significance of the traces, we are now looking forward to hearing the Knox and Sollecito defenses’ counter-arguments.  But as we understand it now, the DNA evidence for the trio having all been involved in the murder seems pretty damning.

The trial to establish the truth about the murder of Meredith continues next Friday. As we’ve reported, various human witnesses have already been heard from: the Postal Police who discovered Meredith’s body, Meredith’s two Italian roommates, and her seven British friends.

Thursday, October 30, 2008

Ground Increasingly Disappearing From Under Knox-Sollecito Defense

Posted by Peter Quennell

Judge Paolo Micheli has now been interviewed by Messaggero Umbria, a newspaper published in Perugia.

The judge really seems to have arrived at a very clear conception of how the cruel, senseless deed took place.  Observe in particular these findings below.

All of them are devastating to the talking-points of Friends Of Amanda recently parroted in dozens of news outlets.

Three attackers were present

I took the opposite approach to that of the defence teams. The lawyers claimed that there was no proof of conspiracy between the three because they didn’t know each other and Kokomani’s testimony wasn’t reliable. They also said that it would have been impossible for them to have organised the crime since they had previous commitments which then fell through. My starting point was the three’s presence in the room where the crime was committed.

DNA on the bra clasp was RS’s

I don’t believe [the bra clasp] was contaminated. The dna either came from outside or it was in the room. It’s not possible that Raffaele Sollecito’s dna was in that room. He had no reason to go there.

No contamination of the knife DNA

It’s true that Amanda’s dna was also on another knife found at Sollecito’s home but there can’t have been contamination. I checked both the objects seized from the cottage in via della Pergola and Sollecito’s apartment in corso Garibaldi. Only once, on Nov6 last year, were objects taken from both locations on the same day and the officers who entered the two buildings were not the same.

Guede was not unknown to other two

The fact that there were no calls [with Rudy] is easy to explain; since Oct27, Rudy hasn’t had a mobile phone. It was taken off him by the police. One of the couple knew Rudy. Meeting people in Perugia is easy, it could have been a chance meeting too.

There was definitely sexual assault

There are some doubts about the dynamics and the position of the victim’s body when she was stabbed. These are however not sufficent to repudiate the hypothesis of sexual assault…. Sexual assault is also an “˜invasion’ of the body as was described in the autopsy. It is certain that the rapist pulled the victim’s top up. Some blood had also run down onto the trousers. It’s therefore plausible to think that whoever violated the victim put their hand down her trousers.

Why there was no rape

Why didnt they complete a rape?] Because she screamed. Also with a knife at her throat and being held down it’s likely that she shouted out. There is a witness, Nara Capezzali, who said she woke up and was shocked by this scream.

Meredith was restrained while taunted

On the victim’s right-hand there was one small cut, a few milimetres long, in between two fingers. On the left-hand, there were four clearly visible cuts. Also the tip of the finger had blood on it. This indicates that the victim’s right-hand was being held as she tried to defend herself with the left. After the fatal stab, she put her hands on the wound.

That last remark really drives home the true horror of Meredith’s incredibly cruel last few minutes. Someone was ferociously slashing away at Meredith like a maniac with a knife. And then did nothing at all to save her.

Walked out on her while she was still alive, clutching her neck to stop the life-blood flowing out of her.

After months of murky semi-silence from police and prosecutors, now the sentencing dossier quoted below and this interview seem like a fire-hose of information.

Is the judge signaling to the defense that a long-form trial will not work to their advantage? That they should simply cave now? Plead guilty, and hope?

And if they don’t, how on earth can they fight THIS sad, sick, depraved stuff?

Monday, October 27, 2008

Sollecito Team Turns Sharply Against Knox? This Is Extraordinary, A Really Big Deal

Posted by Our Main Posters

Breaking News In London Times

A report says Sollecito places Knox at the scene of the crime.

As she had herself as well, twice, in the evening before her arrest. Still, a surprise move coming so soon after this truce.

The report, by Richard Owen from Perugia for the UK Times went online on the Times website three hours ago.

It also confirms what case-watchers already know; that tomorrow, Tuesday, is quite a cliff-hanger for the third defendant, Rudy Guede, who may be convicted and possibly sentenced right there and then.

Amanda Knox, the American flatmate of the murdered British student Meredith Kercher, has for the first time been implicated as being at the scene of the crime by her former Italian boyfriend.

With a verdict imminent in the pre-trial hearings over the murder in Perugia almost a year ago, the three suspects in the case appear to have turned on each other.

After the conclusion of the hearings, Judge Paolo Micheli, 44, a former Carabinieri officer who has been a magistrate since 1990, will decide tomorrow whether Ms Knox and Raffaele Sollecito, her former boyfriend, should stand trial for the murder.

At the same time, he is also due to convict or clear Rudy Guede, the Ivory Coast immigrant who is accused by prosecutors of taking part in the killing, but who has opted for a fast track trial in the hope of a reduced sentence if found guilty.

Lawyers for Mr Sollecito have told the judge that, according to a forensic expert called by the defence, Ms Knox’s DNA is on Ms Kercher’s bloodied bra-strap as well as that of Mr Sollecito and Rudy Guede.

Professor Francesco Vinci, the forensic scientist, said the DNA traces were “too contaminated” to be useable as evidence, but showed the presence of “at least three people”.

The admission appears to support the prosecution case that all three were present at the scene of the crime.

It also breaks a [recent] tacit pact between Ms Knox and Mr Sollecito, who have sent each other supportive letters while in custody and until now have avoided incriminating each other. Mr Sollecito even sent Ms Knox flowers on her birthday this summer.

Lawyers for both Mr Sollecito and Ms Knox have repeatedly claimed the couple spent the night of the murder at Mr Sollecito’s flat, indicating that Mr Guede was the lone killer.

Today, the prosecution and defence lawyers will present their closing arguments. They will argue that if a trial date is set, the suspects should be released from prison into house arrest. Ms Knox has asked to be housed at San Fatucchio, a supervised community and farm in the Umbrian countryside, 40 kilometres from Perugia, for recovering drug addicts and young offenders run by the Catholic charity Caritas.

Last weekend Walter Biscotti, one of Mr Guede’s lawyers, accused Ms Knox and Mr Sollecito of framing his client, a drifter and small-time drug dealer who was brought up in Perugia and mingled with the student community. “We believe Knox and Sollecito were the murderers,” Nicodemo Gentile, another of Mr Guede’s lawyers said.

Mr Biscotti said Mr Guede, the only one of the three who admits he was at the hillside cottage Ms Knox shared with Ms Kercher on the evening of the murder, admitted attempting to have consensual sex with Ms Kercher, but had not raped or killed her. The prosecution says that Mr Guede’s DNA was on Ms Kercher’s bloodstained pillow.

Ms Kercher was found last November semi-naked in her bedroom with her throat cut. The prosecution claims she was assaulted just after Hallo’een in a murderous sex game, possibly inspired by a Japanese comic strip about vampires which Mr Sollecito had been reading.

Prosecutors say that Ms Knox stabbed her flatmate while the other two forced her to her knees and held her down, with Mr Sollecito pinning her by the arms and Mr Guede holding her by the throat.

Ms Knox’s lawyers reject this, saying Ms Kercher was assaulted by “one robust killer”. Last week, Ms Knox burst into tears when the allegation was made in court that she stabbed Ms Kercher, saying: “Meredith was my friend, I had no reason to kill her.”

Mr Guede claims he was listening to his iPod in the bathroom when Ms Kercher was killed in the bedroom. He fled to Germany after the killing, but was tracked down three weeks later in Germany.

Mr Sollecito’s defence team, headed by Giulia Bongiorno, a high profile lawyer and parliamentary deputy, brought props including a shop window mannequin wearing a bra into court last week to back their case. They claim “a thief”, who they suggest was Mr Guede, smashed a window to enter the cottage and killed Ms Kercher when she returned and recognised him, fleeing with her two mobile phones.

Ms Bongiorno argued that the presence of Mr Sollecito’s DNA on the bra fastener but not the rest of the garment proved it was due to contamination and mishandling by police forensic scientists.

Hmmm. Perhaps Rudy Guede should back out of the short-form trial (where the chips are loaded against him but the sentence is guaranteed shorter) and go for the long-form trial instead?

Oh, and better send more flowers, Raffaelle. She is going to be ticked at this one.